raw 264 7 murine macrophage cell line  (ATCC)

Journal: Bioactive Materials

Article Title: Bioengineered titanium implants functionalized with aptamer-valproic acid conjugates orchestrate macrophage programming and mesenchymal stem cell homing for improved osseointegration

doi: 10.1016/j.bioactmat.2026.05.055

Figure Lengend Snippet: A) Live/Dead staining of RAW 264.7 macrophages cultured on different material surfaces for 72 h, where dead cells were stained red and live cells were stained green. B) Cytoskeleton staining morphology of RAW 264.7 grown on different material surfaces for 72 h. C, D, F) Fluorescence images and flow cytometry analysis of intracellular ROS in RAW 264.7 cells with DCFH-DA probe. E) Proliferation of RAW 264.7 macrophages on different material surfaces assessed by CCK-8 assay. n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. Created in BioRender.

Article Snippet: The murine macrophage cell line RAW 264.7 (Procell Life Science, China) was cultured in high-glucose DMEM (Gibco, USA) containing 10% FBS and employed for immunomodulation studies.

Techniques: Staining, Cell Culture, Fluorescence, Flow Cytometry, CCK-8 Assay

Journal: Bioactive Materials

Article Title: Bioengineered titanium implants functionalized with aptamer-valproic acid conjugates orchestrate macrophage programming and mesenchymal stem cell homing for improved osseointegration

doi: 10.1016/j.bioactmat.2026.05.055

Figure Lengend Snippet: A, F) IF staining of iNOS and CD206 in RAW 264.7 macrophages. B-E) Secretion of inflammation-related proteins in RAW 264.7 macrophages. G-J) Relative mRNA expression of inflammation-related genes in RAW 264.7 macrophages. n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The murine macrophage cell line RAW 264.7 (Procell Life Science, China) was cultured in high-glucose DMEM (Gibco, USA) containing 10% FBS and employed for immunomodulation studies.

Techniques: Staining, Expressing

Journal: RSC Advances

Article Title: Salmon bone-derived chondroitin sulfate nanogels for controlled delivery of curcumin: release behavior, structural characteristics and anti-inflammatory efficacy

doi: 10.1039/d6ra06378k

Figure Lengend Snippet: (a) Inhibitory effects on nitric oxide (NO) production and (b) cell viability of RAW 264.7 macrophages treated with blank CS, free curcumin, physical mixture of CS + curcumin (Mix CS + Cur), and CS-Cur nanogel. Data represent mean ± SD ( n = 3, independent replicates). Statistical significance in (a) relative to the curcumin control group is denoted as: * p < 0.05, ** p < 0.01, and **** p < 0.0001.

Article Snippet: For the in vitro cell culture and biological evaluation, the RAW 264.7 murine macrophage cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Control

Journal: Frontiers in Pharmacology

Article Title: Isobavachalcone attenuates experimental periodontitis by suppressing PTGS2-associated macrophage ferroptosis and restoring periodontal immune homeostasis

doi: 10.3389/fphar.2026.1913548

Figure Lengend Snippet: IBC mitigates macrophage ferroptosis via the PTGS2-MAPK-ROS-GPX4 axis. (A–G) Representative Western blots and quantitative analysis of iNOS, PTGS2, p-JNK, JNK, p-p38, p38, GPX4, and IL-1β in LPS-stimulated RAW264.7 cells treated with IBC in a dose-dependent manner. (H, J) Detection and quantification of iNOS, PTGS2, MAPK signaling proteins, and GPX4 in gingival tissues collected from rat models of experimental periodontitis. (I, K) Representative Western blots and quantitative analysis of the PTGS2-MAPK-GPX4 signaling axis in macrophages following OE-PTGS2 plasmid transfection and IBC treatment to assess pathway rescue. (L–P) Microscopic visualization of MitoSOX and FerroOrange signals, accompanied by the measurement of fluorescence intensity and MDA levels in cells undergoing OE-PTGS2 rescue treatments. (Q–T) Representative C11-BODIPY fluorescence imaging, GSH/GSSG ratio, and MDA content assessment in macrophages treated with the ROS scavenger NAC. Data Note: Data are presented as mean ± SD from three independent biological replicates (n = 6) for in vitro experiments, and n = 6 per group for in vivo analysis. Scale bar = 50 μm.

Article Snippet: The RAW264.7 murine macrophage cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, United States of America).

Techniques: Western Blot, Analysis, Plasmid Preparation, Transfection, Fluorescence, Imaging, In Vitro, In Vivo